Rubus ellipticus, a Perennial Weed Host of Prunus Necrotic Ring Spot Virus in India
نویسندگان
چکیده
منابع مشابه
شناسایی و بررسی تنوع ژنتیکی ویروس لکه‌حلقوی بافت مرده هسته‌دارها (Prunus necrotic ring spot virus) در درختان میوه هسته‌دار و دانه‌دار استان کردستان
شمار 149 نمونۀ برگی از درختان میوۀ هسته‌دار و دانه‌دار استان کردستان هنگام بهار و تابستان سال‌های 1394 و 1396 به‌منظور شناسایی مولکولی و بررسی گوناگونی ژنتیکی عامل لکه حلقوی بافت مردۀ درختان هسته‌دار جمع‌آوری شدند و با آغازگرهای اختصاصی ژن پروتئین پوششی PNRSV-F3/PNRSV-R3 آزمون RT-PCR شدند. نتیجهها RT-PCR نشان دادند که 8/20 درصد از نمونه‌ها آلوده به PNRSV بودند. سیزده جدایه بر پایۀ نوع میزبان و...
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Background and Amis: Serological assay is considered as one of the best choices for conducting large number of infection tests. Recombinant DNA technology has been used for expression of virus coat protein (CP) gene in prokaryotic bacterial cells such as Escherichia coli and the recombinant CP (rCP) is used as immunogen in antibody production. Heterologous CP protein expression and purification...
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In recent years, the symptoms of viral diseases such as dwarfism, mosaic, discoloration, necrosis, and circular spots have been prevalent in ornamental plants in parks, gardens, and streets in North Khorasan Province. Tomato ring spot virus (ToRSV) belongs to the Nepovirus genus of Secoviridae family. This virus is a major cause of the infection of ornamental plants in the world. In this study,...
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Cloned cDNA copies of the genomic RNA of melon necrotic spot virus (MNSV) have been sequenced and the sizes and locations of predicted viral proteins have been deduced. The genome consists of at least 4262 nucleotides and the positive strand contains three to five open reading frames (ORFs) which may be expressed. The 5' proximal ORF encodes a 29K protein (p29) and terminates with an amber codo...
متن کاملAnalysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV) using amplicon next generation sequencing
PCR amplicon next generation sequencing (NGS) analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the diversity of the tripartite genome virus, Prunus necrotic ringspot virus (PNRSV) from 53 PNRSV-infected trees using amplicons from conserved g...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 1998
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis.1998.82.11.1283b